VAV3 antibody (AA 566-591)

Catalog No. ABIN210658

This Rabbit Polyclonal antibody specifically detects VAV3 in WB, IHC, ICC and IP. It exhibits reactivity toward Human and Monkey.

Quick Overview for VAV3 antibody (AA 566-591) (ABIN210658)

Target: VAV3 (Vav 3 Oncogene (VAV3))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAV3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-VAV3 Antibody

Binding Specificity: AA 566-591

Specificity: Recognizes Vav3, Mr ~98kD. Additional non-specific bands detected in long exposures at Mr ~38-40kD. A non-specific band at Mr ~95kD was detected in Jurkat lysates. Species cross-reactivity: Human.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Elephant, Dog, Rabbit, Horse (85%) Bat, Pig, Opossum (81%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to aa566-591 (NSGEQGTLKLPEKRTNGLRRTPKQVD) of human Vav3. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Elephant, Dog, Rabbit, Horse (85%), Bat, Pig, Opossum (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IP, WB (0.2 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.2-2 μg/mL of this lot detected Vav3 in RIPA lysates from HeLa and Jurkat cells. Immunohistochemistry: Has been reported by an independent laboratory to detect Vav3 in pancreatic adenocarcinoma sections. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate Vav3 from Jurkat cell lysate and SU86.86 pancreatic tumor cell lysate. Immunocytochemistry: Has been reported by an independent laboratory to detect Vav3 in Jurkat and SU86.86 pancreatic tumor cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Alternative Name: VAV3

Background: Name/Gene ID: VAV3

Synonyms: VAV3, VAV-3, Vav 3 oncogene

Gene ID: 10451

UniProt: Q9UKW4

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway